Count Spankulot is a vampire who exists to spank naughty children who have violated rules. He appears to his victims in a terrifying way and announces their crimes and his name before he proceeds to punish them.
He is a stereotypical vampire character in the vein of Count Dracula, and his powers include those of a typical vampire, such as flight, teleportation and the ability to turn others into vampires. Also like most vampires, he is vulnerable to sunlight and garlic, which is used against him in operations Operation: M.U.N.C.H.I.E.S., Operation: H.O.M.E. and Operation: A.W.A.R.D.S.. Rather than turn him to dust, sunlight just robs Spankula of his powers. Count Spankula speaks with a Romanian accent (as vampires are traditionally believed to be from Transylvania) and is voiced by Daran Norris.
His first appearance was in Operation: C.A.N.N.O.N. where he, among several other villains, attacks the Sector V Treehouse while its defensive systems are down and spanks Numbuhs 2, 3, 4 and 5 for not paying library fines before disappearing in a cloud of smoke.
In his first major appearance, Operation: S.P.A.N.K., it is revealed that Count Spankuat was sent to prison after mistakenly spanking an innocent child, and after being released, resolves to now use his powers to help children rather than punish them. When he sees the Kids Next Door fighting Mr. Wink and Mr. Fibb, forcibly joins Sector V, who make no complaints about this so as not to invoke his wrath. After several missions, in which Count Spankula proves to be more harm than good, the team decides that they must find a way to get rid of him. On a mission one night, they take Count Spankula to a house that they claim belongs to Wink and Fibb and tell him to go inside and spank them. He does so, only to realize that the house actually belongs to the judge who had sentenced him to prison after the previous incident, and that he has been spanking him and his wife. At the trial, the furious judge sentences Spankula to an absurdly long prison term, and the KND apologize to him for this unfortunate "accident". Numbuh 3, not understanding that they are supposed to be lying, then reveals the true nature of the event to Spankula, who then begins furiously spanking them on the spot.
In Operation: L.O.C.K.D.O.W.N., Count Spankula carries out his revenge on the KND by using his hypnotic powers to lure Numbuh 1 to the prison he was incarcerated in, then turning him into a spank-happy vampire like himself, and later, having him do the same to the other Sector V operatives, as well as Numbuh 3's Big Bottom Rainbow Monkey Doll, while the treehouse is on complete lockdown during the night. It is explained here that Count Spankula can turn other people into vampires by spanking the victim with his bare hands, which is why he, along with every other Spank-Happy Vampire, normally wears gloves, creating the Spank-Happy Vampire Army. The only way a victim can cure himself/herself of the Spank-Happy spell is to spank back the vampire that spanked him/her, or spank the original Spank-Happy Vampire (Count Spankula himself), which is how the members of Sector V return to normal at the end of the episode. In Operation: V.I.D.E.O.G.A.M.E., Count Spankula turns the KND into vampires much like in Operation: L.O.C.K.D.O.W.N. (only Numbuh 5 is transformed instead of Numbuh 4) and is fought as a boss in the level Operation: S.P.A.N.K.A.R.I.F.F.I.C..
In Operation: S.P.A.N.K.E.N.S.T.I.N.E., before he could administer a spanking to Mushi Sanban for "murdering" Numbuh 3's Posh Party Rainbow Monkey Doll in Operation: C.L.U.E.S., Mushi used an evil machine on him to revive the Rainbow Monkey Doll as an undead monster. He is later seen at the Sanban's dinner party, reminiscing on the old days with Lydia Gilligan.
- He is definitely the only male villain in the entire KND franchise to be based on a Mythical Creature, while all of the other male villains are humans.
- He is the only villian to face any legal repercussions for his actions.